The Metabolism of Lactate and Pyruvate by Pseudomonas AM1

Metabolism of pyruvate and L-lactate by rat adipose tissue.

The metabolism of r-lactate and pyruvate, labeled uniformly with 14C, by epididymal fat pad tissue of fasted and fasted-refed rats was studied. In tissue of fasted-refed rats, pyruvate and L-lactate were utilized at equal rates. L-Lactate was nearly all converted into CO2 and fatty acids, and pyruvate into COZ, fatty acids, and L-lactate. The incorporation of 14C into glycerol was very low. L-L...

Efficient Production of Pyruvate from DL-Lactate by the Lactate-Utilizing Strain Pseudomonas stutzeri SDM

BACKGROUND The platform chemical lactate is currently produced mainly through the fermentation of sugars presented in biomass. Besides the synthesis of biodegradable polylactate, lactate is also viewed as a feedstock for the green chemistry of the future. Pyruvate, another important platform chemical, can be produced from lactate through biocatalysis. METHODOLOGY/PRINCIPAL FINDINGS It was est...

Lactate and pyruvate metabolism in the exercising ischemic limb.

Uptake of methylamine and methanol by Pseudomonas sp. strain AM1.

The uptake of methylamine and of methanol by the facultative methylotroph Pseudomonas sp. strain AM1 was investigated. It was found that this organism possesses two uptake systems for methylamine. One of these operates when methylamine is the sole source of carbon, nitrogen, and energy. It has a Km of 1.33 X 10(-4) M and a Vmax of 67 nmol/min per mg of cells (dry weight). The other system, foun...

Lactate dehydrogenase isoenzymes a comparison of pyruvate-to-lactate and lactate-to-pyruvate assays.

Both assays are accepted reference methods for measurement of lactate dehydrogenase (LDH) activity (2-4). At pH 7.4, pyruvate -* lactate (P -* L) activity is about three times lactate -+ pyruvate (L P) activity, because equilibrium favors formation of lactate. Normal range for the P -* L assay is 130-265 spectrophotometric units at 25#{176} (3); normal range for the L P assay is 40-98 units at ...